间接荧光抗体法快速诊断海兰褐蛋鸡J亚群禽白血病的研究Rapid diagnosis of avian leukosis subgroup J of egg-type chicken by indirect fluorescent assay
徐镔蕊,董卫星,何召庆,冯小宇,余春明,张丽,任艳丽,L F LEE,Mao-xiang LI
摘要(Abstract)：
某地区海兰褐蛋鸡发生肿瘤性传染病 ,经临床症状和病理学检查 ,在肝、脾、肾、卵巢、输卵管、肺、骨髓、腺胃、肠道等可见胞浆内充满球形嗜酸性颗粒的骨髓瘤细胞 ,呈灶状或弥漫性的分布。该变化是禽骨髓细胞瘤病病理学的特征性变化 ,具有证病意义。经用特异性抗 J亚群白血病病毒囊膜蛋白 gp85的单克隆抗体的免疫组化方法 ,在病料组织切片上检出病毒抗原 ,从而确诊为蛋鸡 J亚群禽白血病。本研究是尝试用间接荧光抗体法来快速诊断该病。采用特异性抗 J亚群禽白血病病毒囊膜蛋白 gp85单克隆抗体对组织触片作间接荧光抗体试验 ,检测 J亚群禽白血病病毒抗原。在骨髓、食管、肌肉、输卵管、肾都出现了范围广而且很明亮的荧光 ;肝、肺、脾的荧光较为明亮 ;心脏的荧光较弱 ,但清晰可见。表明在病料组织中存在特异性病毒抗原。
关键词(KeyWords)： J亚群禽白血病;蛋鸡;间接荧光试验;单克隆抗体
基金项目(Foundation):
作者(Author): 徐镔蕊,董卫星,何召庆,冯小宇,余春明,张丽,任艳丽,L F LEE,Mao-xiang LI
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参考文献(References)：
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