陈大伟;刘敏;唐修君;陆俊贤;赵敏;刘茵茵;马丽娜;周倩;王娟;高玉时;
CHEN Da-wei;LIU Min;TANG Xiu-jun;LU Jun-xian;ZHAO Min;LIU Yin-yin;MA Li-na;ZHOU Qian;WANG Juan;GAO Yu-shi;Jiangsu Institute of Poultry Science;Jiangsu Weitekai Pigeon Industry Co., Ltd.;Jiangdu District Wujian Town Bureau of Agriculture and Rural Affairs;

• 1:江苏省家禽科学研究所
• 2:江苏威特凯鸽业有限公司
• 3:江都区武坚镇农业农村局

摘要(Abstract)：

为了探索亲鸽哺育乳鸽期间使用地美硝唑后亲鸽和乳鸽体内地美硝唑(DMZ)残留分布及代谢规律，制定适合肉鸽生产的休药期规范。本试验于亲鸽开始哺乳第1天以拌料和灌胃2种方式投喂DMZ,停药后不同时间采集亲鸽、乳鸽组织和鸽乳，采用液相色谱串联质谱法测定DMZ及其代谢物残留量。结果显示，亲鸽采用饲喂和灌胃2种方式投喂DMZ后第1天亲鸽肌肉、肝脏、肾脏和皮脂中DMZ及其代谢物残留量均达到峰值，灌胃方式给药后第1天皮脂中DMZ及其代谢物含量高达6 591.7μg/kg,随后各组织残留量逐渐下降；饲喂方式给药后亲鸽肌肉、肝脏、肾脏和皮脂中药物消除时间分别为16 d、19 d、19 d和19 d,灌胃方式下分别为22 d、22 d、19 d和19 d;乳鸽各组织中药物残留代谢规律与亲鸽基本类似，最高残留量是灌胃后乳鸽皮脂组织，为500.7μg/kg;饲喂方式给药后乳鸽肌肉、肝脏、肾脏和皮脂中药物消除时间分别为16 d、16 d、16 d和13 d,灌胃方式下分别为13 d、19 d、16 d和13 d; 2种给药方式下第10天鸽乳中均无药物检出。结果表明，亲鸽使用DMZ,饲喂方式给药建议亲鸽休药时间为19 d,乳鸽为16 d;灌胃方式给药建议亲鸽休药时间为22 d,乳鸽为19 d。
This study investigated the residual distribution and metabolic regularity of dimetridazole(DMZ) in parent and squab pigeons after the use of DMZ during the feeding period of pigeons, and formulated the standard of withdrawal time suitable for meat pigeon production. On the first day of lactation, parent pigeons were given DMZ in two ways: feeding and gavage, and the tissues of parent and squab pigeons and pigeon milk were collected at different times to determine the DMZ residue and its metabolites by liquid chromatography-mass spectrometry. The results showed that the DMZ residue and its metabolites in muscle, liver, kidney and sebum, of which parent pigeons were fed with DMZ by feeding and gavage, reached the peak on the first day. The DMZ residue and its metabolites in sebum was up to 6 591.7 μg/kg on the first day by gavage, and then gradually decreased in each tissue. The drug elimination times of DMZ in muscle, liver, kidney and sebum were 16 days, 19 days, 19 days and 19 days, respectively, by feeding, but 22 days, 22 days, 19 days, and 19 days by gavage. The metabolic regularity of DMZ residue in squab tissues were similar to those of their parents. The maximum DMZ residue was 500.7 μg/kg in sebum by gavage. The elimination times of DMZ in muscle, liver, kidney and sebum of squabs were 16 days, 16 days, 16 days and 13 days by feeding, but 13 days, 19 days, 16 days and 13 days by gavage, respectively. No DMZ was detected in pigeon milk on day 10 by two administration methods. It is thus clear that the withdrawal times are 19 days for parent pigeons and 16 days for squabs by feeding, while the withdrawal times are 22 days for parent pigeons and 19 days for squabs by gavage, after using DMZ for parent pigeons.

关键词(KeyWords)： 地美硝唑;鸽;残留;代谢规律
dimetridazole;pigeon;residue;metabolic regularity

Abstract:

Keywords:

基金项目(Foundation): 江苏现代农业产业技术体系建设专项资金(JATS[2022]402);; 江苏省农业科技自主创新资金[CX(21)2011];; 江苏省种业振兴揭榜挂帅项目[JBGS(2021)109]

作者(Authors): 陈大伟;刘敏;唐修君;陆俊贤;赵敏;刘茵茵;马丽娜;周倩;王娟;高玉时;
CHEN Da-wei;LIU Min;TANG Xiu-jun;LU Jun-xian;ZHAO Min;LIU Yin-yin;MA Li-na;ZHOU Qian;WANG Juan;GAO Yu-shi;Jiangsu Institute of Poultry Science;Jiangsu Weitekai Pigeon Industry Co., Ltd.;Jiangdu District Wujian Town Bureau of Agriculture and Rural Affairs;

参考文献(References)：

• [1] 卜柱，厉宝林，赵振华，等.中国肉鸽主要品种资源与育种现状[J].中国畜牧兽医，2010,37(6):116- 119.
• [2] 边世雄，孙研研，麻慧，等.鸽乳的组成及其生成调控机制的研究进展[J].畜牧兽医学报，2020,51(9):2048- 2058.
• [3] 倪爱心.鸽毛滴虫抗性基因筛选及虫源外泌体蛋白质谱分析[D].北京：中国农业科学院，2020.
• [4] 农业部.动物性食品中兽药最高残留限量[Z].农业部公告[2002]235号.北京：农业部，2002-12-24.
• [5] 农业部.兽药停药期规定[Z].农业部公告[2003]278号.北京：农业部，2003-05-23.
• [6] 富玥.高效液相色谱-串联质谱法检测猪肝中硝基咪唑类药物残留量[J].现代食品，2022,28(3):171- 175.
• [7] 张静，高玉时，周倩，等.高效液相色谱-串联质谱法同时测定鸡肉和鸡蛋中10种硝基咪唑类药物及代谢物[J].分析科学学报，2021,37(2):217- 222.
• [8] 龙玉华，廖梅.鸽毛滴虫病的防治[J].贵州畜牧兽医，2016,40(4):45.
• [9] Qiu S B,Lv M N,Xi H,et al.PCR identification and phylogenetic analysis of trichomonas gallinae from domestic pigeons in Guangzhou,China[J].Korean Journal of Parasitology,2017,55(3):333- 336.
• [10] 姜夕玥.鸽毛滴虫粘附蛋白AP65的克隆、鉴定及表面定位[D].泰安：山东农业大学，2015.
• [11] 单达聪，于肖寒，计峰，等.北京、河北及山东部分地区鸽毛滴虫感染情况的调查[J].中国家禽，2019,41(11):74- 76.
• [12] 陈海华，薛勇，金李萍，等.蛇床子素体外抗鸽毛滴虫的效果及形态学观察[J].畜牧与兽医，2017,49(4):91- 94.
• [13] 罗锋，陈泽华，苏遂琴，等.鸽毛滴虫病的研究进展[J].中国兽医寄生虫病.2007(3):51- 54.
• [14] Spriggs M C,Purple K E,Gerhold R W.Detection of Trichomonas gallinae in wild birds admitted to a rehabilitation center,Florida,USA[J].J Wildl Dis,2020,56(3):733- 735.
• [15] Martinez-Herrero M C,Sansano-Maestre J,Ortega J,et al.Oral trichomonosis:description and severity of lesions in birds in Spain[J].Vet Parasitol,2020,283:109196.
• [16] 黄名英，廖党金，文红，等.四川鸽毛滴虫病的调查报告[J].中国家禽，2008,30(5):56- 57.
• [17] 罗锋，翁亚彪，张腱菲，等.广东省鸽毛滴虫感染情况的调查[J].中国家禽，2006(17):24- 25.
• [18] 孙炎戊，陈志，陈思彤，等.广东省鸽毛滴虫感染流行调查[J].养禽与禽病防治，2020(10):5- 9.
• [19] Ding J M,Liao N,Zheng Y M,et al.The composition and function of pigeon milk microbiota transmitted from parent pigeons to squabs[J].Front Microbiol,2020,11:1789.
• [20] 梁勇，姜庆林，陈益填，等.鸽乳成分及其分泌机制研究进展[J].中国家禽，2016,38(20):47- 51.