周佳;许倩倩;杨跃飞;王彦红;
ZHOU Jia;XU Qian-qian;YANG Yue-fei;WANG Yan-hong;College of Veterinary Medicine, Yangzhou University;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University;Key Laboratory of Human-Animal Pathology of Jiangsu Province;

• 1:扬州大学兽医学院
• 2:扬州大学江苏高校动物重要疫病与人兽共患病防控协同创新中心
• 3:江苏省人兽共患病学重点实验室

摘要(Abstract)：

为调查不同动物源性大肠杆菌的耐药性，本试验对65株源于畜禽的大肠杆菌进行18种常见抗菌药的药敏试验，用PCR法检测3种氨基糖苷类耐药基因aac(3)-II、arm(A)和aac(6′)-Ib。结果显示：65株大肠杆菌中对氨基糖苷类药物链霉素耐药率最高(75.38%),其次为复方新诺明(69.23%),耐药率最低的是头孢哌酮舒巴坦(0%);耐药基因aac(3)-II、arm(A)和aac(6′)-Ib的检出率分别为29.23%、1.54%和9.23%;有51株分离菌株存在多重耐药，占总株数的78.46%。结果表明，动物源大肠杆菌对多种抗菌药具有耐药性，在氨基糖苷类耐药基因中以携带aac(3)-II为主。
In order to investigate the drug resistance in Escherichia coli(E. coli) of animal origins, this study performed drug susceptibility test on 65 E. coli isolates from livestock and poultry using 18 common antibacterial agents, and detected three aminoglycoside resistance genes including aac(3)-II, arm(A) and aac(6′)-Ib by PCR. The results showed that among 65 E. coli isolates, 75.38% were resistant to streptomycin belonging to aminoglycosides, 69.23% were resistant to compound sulfamethoxazole, and 0% was resistant to cefoperazone sulbactam; the resistance genes aac(3)-II, arm(A) and aac(6′)-Ib were found in 29.23%, 1.54% and 9.23% of E. coli isolates; and 51(78.46%) E. coli isolates were multi-drug resistant. Thus, E. coli isolates of animal origins are resistant to a variety of antimicrobials, and aac(3)-II gene is mainly carried among the aminoglycosides resistance genes.

关键词(KeyWords)： 大肠杆菌;氨基糖苷;药敏试验;耐药基因
Escherichia coli;aminoglycoside;drug sensitivity test;drug resistance gene

Abstract:

Keywords:

基金项目(Foundation): 江苏高校品牌专业建设工程资助项目(PPZY2015B158);; 江苏高校优势学科建设工程资助项目;; 扬州大学大学生创新创业训练计划基金(X20190682);; 江苏省人兽共患病学重点实验室资助项目(Rl708)

作者(Authors): 周佳;许倩倩;杨跃飞;王彦红;
ZHOU Jia;XU Qian-qian;YANG Yue-fei;WANG Yan-hong;College of Veterinary Medicine, Yangzhou University;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University;Key Laboratory of Human-Animal Pathology of Jiangsu Province;

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